According to the Doctor, when testing the crew, Voyager has 128 crew members on board, including himself.
Actor Tucker Smallwood portrayed Admiral Bullock while suffering from the paralyzing condition known as Bell's palsy.
Early in the episode, when Chakotay meets Commander Archer in the bar, there is a sign on the wall behind them simply entitled "Starfleet Ships." Four of the five ship silhouettes displayed are former classes of the USS Enterprise - Constitution, Constitution Refit (Enterprise A), Excelsior and Galaxy. The Fifth, in the bottom right, is the rarely seen Norway Class.
Captain Janeway usually sits at the head of the table in the meeting room but during the meeting with Species 8472, she sits across the table from their leader, Boothby. This was intentional, as it shows that the captain considers herself and Boothby to be equal to each other as they are both leaders of their groups.