In the scene in which Whitney Frost is being directed on the fictional set of her latest film, many of Agent Carter's real crew members donned 1940's clothing to appear as extras.
The writers originally wanted to call the Zero Matter 'Element Zero', but consultant and physicist Clifford V. Johnson quickly pointed out to them that there actually is a substance named element 0: neutronium.
Adam Hart served as the stunt double for James Darcy in a few situations.
The name of Chadwick's project "Isodyne" is a near-homophone of "ice-nine", which is a deadly form of water that freezes at room temperature from the Kurt Vonnegut Jr. novel "Cat's Cradle".